| Title | Complete enzymic digestion of acidic proteins. |
| Publication Type | Journal Article |
| Year of Publication | 1976 |
| Authors | Kay J |
| Journal | Int J Pept Protein Res |
| Volume | 8 |
| Issue | 4 |
| Pagination | 379-83 |
| Date Published | 1976 |
| ISSN | 0367-8377 |
| Keywords | Amino Acids, Carboxypeptidases, Dipeptidases, Fibrinogen, Gastrins, Hydrogen-Ion Concentration, Hydrolysis, Leucyl Aminopeptidase, Pepsinogens, Ribonucleases, Subtilisins |
| Abstract | Acidic proteins are usually resistant to complete enzymic hydrolysis. The increasing number of "unusual" amino acids, which are unstable to acid hydrolysis, makes it necessary to have a method of enzymic hydrolysis applicable to all proteins. The complete hydrolysis of four acidic proteins by subtilisin plus leucine amino-peptidase plus prolidase followed by carboxypeptidase C, is described. Recoveries of amino acids were in excellent agreement with the expected content from the known sequences. |
| DOI | 10.1111/j.1399-3011.1976.tb02517.x |
| Alternate Journal | Int J Pept Protein Res |
| PubMed ID | 8400 |