| Title | Biochemical characterization of the tartrate-resistant acid phosphatase of human spleen with leukemic reticuloendotheliosis as a pyrophosphatase. |
| Publication Type | Journal Article |
| Year of Publication | 1977 |
| Authors | Lam KW, Yam LT |
| Journal | Clin Chem |
| Volume | 23 |
| Issue | 1 |
| Pagination | 89-94 |
| Date Published | 1977 Jan |
| ISSN | 0009-9147 |
| Keywords | Acid Phosphatase, Binding, Competitive, Humans, Hydrogen-Ion Concentration, Kinetics, Lymphatic Diseases, Molecular Weight, Pyrophosphatases, Spleen, Splenic Neoplasms, Tartrates |
| Abstract | A tartrate-resistant acid phosphatase was isolated from a human leukemic spleen by freeze-thawing in saline and purified by repeated chromatography on carboxymethyl-cellulose. The purified enzyme has a molecular weight of 64 000. It catalyzes the hydrolysis of inorganic and organic pyrophosphate as well as the phenolic ester of monoorthophosphate, with optimal activity between pH 5 and 6. However, there is no activity toward mono-orthophosphate esters of aliphatic alcohols. The present data have identified its catalytic function as a pyrophosphatase. However, it has properties different from the pyrophosphatase previously observed in normal animal tissues. |
| Alternate Journal | Clin Chem |
| PubMed ID | 12883 |