| Title | Characterization of monohydroxylated lipoxygenase metabolites of arachidonic and linoleic acid in rabbit peritoneal tissue. |
| Publication Type | Journal Article |
| Year of Publication | 1982 |
| Authors | Claeys M, Coene MC, Herman AG, Jouvenaz GH, Nugteren DH |
| Journal | Biochim Biophys Acta |
| Volume | 713 |
| Issue | 1 |
| Pagination | 160-9 |
| Date Published | 1982 Oct 14 |
| ISSN | 0006-3002 |
| Keywords | Animals, Arachidonic Acids, Chemical Phenomena, Chemistry, Hydroxyeicosatetraenoic Acids, Isomerism, Linoleic Acid, Linoleic Acids, Linoleic Acids, Conjugated, Lipoxygenase, Peritoneum, Rabbits |
| Abstract | Rabbit peritoneal tissue contains a lipoxygenase which converts arachidonic acid preferentially into 15-hydroxy-5,8,11,13-eicosatetraenoic acid. Stereochemical analysis of the menthyloxycarbonyl derivative of this metabolite by means of a high-pressure liquid chromatography method, involving the use of a Ag+ -loaded cation-exchange column, indicated that it has mainly the 15-Ls-hydroxy configuration. The biosynthesis of 15-hydroxy-5,8,11,13-eicosatetraenoic acid could be confirmed during examination of the monohydroxy acids obtained without addition of fatty acids, thus formed from endogenously released substrate. However, the 9-and 13-hydroxy derivatives of linoleic acid were also formed and in quantities exceeding those of 15-hydroxy-5,8,11,13-eicosatetraenoic acid. |
| DOI | 10.1016/0005-2760(82)90178-3 |
| Alternate Journal | Biochim Biophys Acta |
| PubMed ID | 6814493 |