| Title | Distribution of lysosome populations in rat cardiac tissue. |
| Publication Type | Journal Article |
| Year of Publication | 1975 |
| Authors | Smith AL, Bird JW |
| Journal | Recent Adv Stud Cardiac Struct Metab |
| Volume | 7 |
| Pagination | 41-8 |
| Date Published | 1975 |
| ISSN | 0363-5872 |
| Keywords | Adenosine Triphosphatases, Animals, Catalase, Cell Fractionation, Centrifugation, Zonal, Glucuronidase, Hydrogen-Ion Concentration, Kinetics, Lysosomes, Myocardium, Peptide Hydrolases, Rats |
| Abstract | The post-nuclear fraction of rat heart tissue was fractionated by isopycnic zonal centrifugation in sucrose gradients, followed by differential centrifugation of the zonal fractions (rho-S fractionation). The distribution of 5 lysosomal acid hydrolases, a protease with neutral and alkaline activity and several marker enzymes for cell organelles (catalase, Ca2+-ATPase, cytochrome oxidase, glucose-6-phosphatase and muramidase) were studied. Three major lysosomal populations were described with equilibrium densities of 1.09, 1.17, and 1.23 gms cc-1 (omega2t = 1.54 X 10(11) rad2 sec-1), and a continuum in the size of these particles at the three different densities. |
| Alternate Journal | Recent Adv Stud Cardiac Struct Metab |
| PubMed ID | 5760 |