Mechanical precipitation of hemoglobin köln.

TitleMechanical precipitation of hemoglobin köln.
Publication TypeJournal Article
Year of Publication1975
AuthorsAsakura T, Adachi K, Shapiro M, Friedman S, Schwartz E
JournalBiochim Biophys Acta
Volume412
Issue2
Pagination197-201
Date Published1975 Dec 15
ISSN0006-3002
KeywordsChemical Precipitation, Germany, West, Hemoglobin, Sickle, Hemoglobins, Abnormal, Humans, Hydrogen-Ion Concentration, Methionine, Osmolar Concentration, Temperature, Valine
Abstract

Hb Köln (beta 98 Val leads to Met) was found to precipitate rapidly during mechanical shaking. The rate of precipitation of Hb Köln is 5-6 times faster than that of Hb S. The kinetics of precipitation of the patient's hemolysate, which is a mixture of Hb Köln and Hb A, showed a biphasic curve indicating that Hb Köln precipitates independently from Hb A. The instability of Hb Köln may be attributed to the conformational change in the vicinity of heme. The mechanical shaking may be used as a new method for detection and quantitation of hemoglobin Köln and other unstable hemoglobins.

DOI10.1016/0005-2795(75)90034-3
Alternate JournalBiochim Biophys Acta
PubMed ID83

Weill Cornell Medicine Neurology 525 E. 68th St.
PO Box 117
New York, NY 10065 Phone: (212) 746-6575