| Title | Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification. |
| Publication Type | Journal Article |
| Year of Publication | 1975 |
| Authors | Camici G, Manao G, Ramponi G |
| Journal | Physiol Chem Phys |
| Volume | 7 |
| Issue | 5 |
| Pagination | 409-14 |
| Date Published | 1975 |
| ISSN | 0031-9325 |
| Keywords | Adenosine Monophosphate, ATP Citrate (pro-S)-Lyase, Enzyme Activation, Hydrogen-Ion Concentration, Kinetics, Klebsiella, Oxo-Acid-Lyases |
| Abstract | The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme. |
| Alternate Journal | Physiol Chem Phys |
| PubMed ID | 698 |