Role of cellular density, in vitro, in anti-tumor activity of CFA-treated and immunized cells.

TitleRole of cellular density, in vitro, in anti-tumor activity of CFA-treated and immunized cells.
Publication TypeJournal Article
Year of Publication1976
AuthorsUyeki EM, Truitt GA, Bisel TU
JournalAgents Actions
Volume6
Issue6
Pagination694-700
Date Published1976 Nov
ISSN0065-4299
KeywordsAnimals, Antineoplastic Agents, Cell Count, Cells, Cultured, DNA, Neoplasm, Female, Freund's Adjuvant, Goats, Immunization, Macrophages, Mice, Mice, Inbred C57BL, Mice, Inbred DBA, Neoplasms, Experimental, Rabbits, Thymidine
Abstract

Incorporation of tritiated deoxythymidine (3HdT) into DNA was used to measure growth, in vitro, of P815 tumor cells admixed with spleen and peritoneal effector cells. At a high tumor cell density ((1x10(5) cells per dish), using anti-theta and anti-macrophage sera, T-cells and macrophages from the peritoneum of immunized mice could be identified as cells possessing anti-tumor activity. A nonspecific inhibition by normal effector cells, which occurred at the high tumor cell density, did not occur at a lower tumor cell density (1x10(4) cells per dish). Therefore, the effects of immunization and Freund's adjuvant treatment on the anti-tumor activity of effector cells were determined more accurately when normal cells were no longer inhibitory. Thus, experimental variables dealing with cellular density (cells/mm2 of the culture vessel surface) and effector:tumor cell ratios play an important role in the anti-proliferative capacity of effector cells.

DOI10.1007/BF02026090
Alternate JournalAgents Actions
PubMed ID1008014

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